- GENE CONSTRUCTION KIT ERROR 199 MANUAL
- GENE CONSTRUCTION KIT ERROR 199 SOFTWARE
- GENE CONSTRUCTION KIT ERROR 199 CODE
- GENE CONSTRUCTION KIT ERROR 199 PC
- GENE CONSTRUCTION KIT ERROR 199 MAC
I thought the files could be transferred between operating systems:
GENE CONSTRUCTION KIT ERROR 199 MAC
I'm having difficulties opening Mac GCK files on a Windows system. GCK will then search the sequence starting from the cursor and will highlight the next occurrence of that sequence in the sequence listing itself. From here you basically have two options:įor short DNA segments, simply type in the bases you wish to search for then select 'Search'.įor larger stretches - you can use the Shortcut Keys - for the copy/paste commands - to copy the text from the known segment, and pasting into the Search dialog.
GENE CONSTRUCTION KIT ERROR 199 MANUAL
23 in the current GCK manual describes the process for "Finding a Sequence".īegin by activating the window of the construct in question and then selecting "Find Sequence" from the Construct Menu. How do I search a construct for a known sequence of DNA from another source: If the feature is set to 'Hide' then its menu option will not be available. The results will be the same for the other options in the Display menu: Show / Hide Regions, Frames, Sites, Introns, Primers, Comments, and Generations (if applicable for the active construct file).
![gene construction kit error 199 gene construction kit error 199](https://ars.els-cdn.com/content/image/1-s2.0-S2001037020303226-ga1.jpg)
Now the 'Mark Sites' menu option should again be available (and any previously marked sites should appear on your construct). The fix is simply to choose Construct -> Display -> Show Sites. Once you've chosen to 'Hide Sites', GCK will not allow you to 'Mark' new sites because they would essentially be invisible to you - causing confusion (and sometimes rage)! Most likely the option to 'Hide Sites' was selected (perhaps inadvertently) from the Construct -> Display menu. I have a plasmid in which I can't 'Mark Sites' - the menu item is grayed out - what's going on: Once you have moved all your favorite enzymes into your new list file, simply save it in the "GCK Data" folder, and it will be available in the drop down menu next time you choose to 'Mark Sites'. Note - To speed up the process, you can select multiple enzymes at a time by holding down the Ctrl/Command key while selecting them - then simply Ctrl/Command+C Ctrl/Command+V into your new List file. Use the Shortcut Keys - for the copy/paste commands - as sometimes the clipboard gets confused when working in these types of windows. simply highlight the enzymes you are most interested in from the GCK file by single clicking on the enzyme name, and then "Copy" and "Paste" them into your new list file. Now, you can open up the enzyme list files that are included with GCK from which you want to move enzymes into your new file - just select "Open" from the File menu, look in the "GCK Data" folder, and click on the "List" radio button (this will display only those enzyme list files). Name it something like "My Enzymes" - (or whatever you prefer) What you'll need to do is first create a new List window by selecting "New" from the File menu, and then choosing the "List" radio button.
![gene construction kit error 199 gene construction kit error 199](https://d2vlcm61l7u1fs.cloudfront.net/media%2F5c7%2F5c79e97d-93cc-4375-bb7f-49860c7f2314%2Fphps6Crv6.png)
These are the files for storing enzymes used in the "Mark Sites" and "List Sites" feature available in the Construct menu. Is there a way to pick some enzymes from the lists available in GCK and make my own list? That way I can just go to "My list" and add all when marking restriction enzyme sites in my constructs:Ĭhapter 4 in the GCK manual describes what we call "List Windows".
GENE CONSTRUCTION KIT ERROR 199 SOFTWARE
GENE CONSTRUCTION KIT ERROR 199 CODE
![gene construction kit error 199 gene construction kit error 199](https://screenshots.macupdate.com/JPG/17031/17031_1564397829_scr.jpg)
Where did they go - and how do I get them back?